A Novel Luminescence-Based High-Throughput Approach for Cellular Resolution of Protein Ubiquitination Using Tandem Ubiquitin Binding Entities (TUBEs).

Akinjiyan, Favour A; Fazal, Aleem; Hild, Marc; Beckwith, Rohan E J; Ross, Nathan T; Paulk, Joshiawa; Carbonneau, Seth

Akinjiyan, Favour A; Fazal, Aleem; Hild, Marc; Beckwith, Rohan E J; Ross, Nathan T; Paulk, Joshiawa; Carbonneau, Seth.

Afiliação

Akinjiyan FA; Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
Fazal A; Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
Hild M; Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
Beckwith REJ; Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
Ross NT; Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
Paulk J; Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
Carbonneau S; Chemical Biology and Therapeutics, Novartis Institutes for Biomedical Research, Cambridge, MA, USA.

SLAS Discov ; 25(4): 350-360, 2020 04.

Article em En | MEDLINE | ID: mdl-31997692

Assuntos

Descoberta de Drogas; Ensaios de Triagem em Larga Escala; Complexo de Endopeptidases do Proteassoma/genética; Ubiquitina/genética; Humanos; Luminescência; Ligação Proteica/genética; Transporte Proteico/genética; Ubiquitinação/genética

Palavras-chave

G to S Phase Transition 1 Protein (GSPT1); TUBE; cellular assay; luminescence; ubiquitin

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ubiquitina / Complexo de Endopeptidases do Proteassoma / Descoberta de Drogas / Ensaios de Triagem em Larga Escala Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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