Antibodies against purified epithelial sodium channel protein from bovine renal papilla.
Am J Physiol
; 255(6 Pt 1): C835-43, 1988 Dec.
Article
em En
| MEDLINE
| ID: mdl-3202153
ABSTRACT
Monoclonal and polyclonal antibodies against amiloride-sensitive sodium channel protein purified from bovine renal papilla have been produced. These antibodies show considerable specificity in enzyme-linked immunosorbent assay (ELISA) and dot-blot assays and can immunoprecipitate radiolabeled channel protein. The polyclonal antibodies bind two sodium channel subunits on Western blots, namely, the 300- and 110-kDa polypeptides, and cross react with channel protein isolated from the amphibian A6 renal epithelial cell line. They can be used to immunoaffinity purify the channel in relatively high yield from a crude, detergent-solubilized bovine kidney homogenate. These antibodies should be useful in isolating the sodium channel gene, in studying the channel protein structure, in studying immunocytochemical localization, and in allowing purification of the channel from other tissue sources.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Sódio
/
Medula Renal
/
Proteínas de Membrana
/
Anticorpos
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article