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Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.
Luo, Lin; Ambrozkiewicz, Mateusz C; Benseler, Fritz; Chen, Cui; Dumontier, Emilie; Falkner, Susanne; Furlanis, Elisabetta; Gomez, Andrea M; Hoshina, Naosuke; Huang, Wei-Hsiang; Hutchison, Mary Anne; Itoh-Maruoka, Yu; Lavery, Laura A; Li, Wei; Maruo, Tomohiko; Motohashi, Junko; Pai, Emily Ling-Lin; Pelkey, Kenneth A; Pereira, Ariane; Philips, Thomas; Sinclair, Jennifer L; Stogsdill, Jeff A; Traunmüller, Lisa; Wang, Jiexin; Wortel, Joke; You, Wenjia; Abumaria, Nashat; Beier, Kevin T; Brose, Nils; Burgess, Harold A; Cepko, Constance L; Cloutier, Jean-François; Eroglu, Cagla; Goebbels, Sandra; Kaeser, Pascal S; Kay, Jeremy N; Lu, Wei; Luo, Liqun; Mandai, Kenji; McBain, Chris J; Nave, Klaus-Armin; Prado, Marco A M; Prado, Vania F; Rothstein, Jeffrey; Rubenstein, John L R; Saher, Gesine; Sakimura, Kenji; Sanes, Joshua R; Scheiffele, Peter; Takai, Yoshimi.
Afiliação
  • Luo L; Djavad Mowafaghian Centre for Brain Health and Department of Psychiatry, University of British Columbia, 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada.
  • Ambrozkiewicz MC; Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany; Institute of Cell Biology and Neurobiology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Ber
  • Benseler F; Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.
  • Chen C; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China.
  • Dumontier E; Department of Neurology & Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada.
  • Falkner S; Biozentrum of the University of Basel, Basel, Switzerland.
  • Furlanis E; Biozentrum of the University of Basel, Basel, Switzerland.
  • Gomez AM; Biozentrum of the University of Basel, Basel, Switzerland.
  • Hoshina N; F.M. Kirby Neurobiology Center, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Huang WH; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montreal, QC H3G 1A4, Canada.
  • Hutchison MA; Synapse and Neural Circuit Research Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
  • Itoh-Maruoka Y; Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
  • Lavery LA; Department of Molecular and Human Genetics, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX 77003, USA; Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA.
  • Li W; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China.
  • Maruo T; Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan; Department of Biochemistry, Tokushima University Graduate School of Medical Sciences, 3-18-15, Kuramo
  • Motohashi J; Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
  • Pai EL; Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, UCSF Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA 94158, USA; Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Pelkey KA; Section on Cellular and Synaptic Physiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
  • Pereira A; Department of Neurobiology and Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA.
  • Philips T; Department of Neurology and Brain Science Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Sinclair JL; Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
  • Stogsdill JA; Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02139, USA.
  • Traunmüller L; Biozentrum of the University of Basel, Basel, Switzerland.
  • Wang J; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  • Wortel J; Department of Functional Genomics and Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and University Medical Center Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.
  • You W; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA; Departments of Genetics, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
  • Abumaria N; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai 200032, China; Department of Laboratory Animal Science, Shanghai Medical College, Fudan University, Shanghai 200032, China.
  • Beier KT; Department of Physiology and Biophysics, Center for the Neurobiology of Learning and Memory, University of California, Irvine, Irvine, CA 92697, USA.
  • Brose N; Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.
  • Burgess HA; Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
  • Cepko CL; Departments of Genetics, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
  • Cloutier JF; Department of Neurology & Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada.
  • Eroglu C; Department of Cell Biology, Department of Neurobiology, and Duke Institute for Brain Sciences, Regeneration Next Initiative, Duke University Medical Center, Durham, NC 27710, USA.
  • Goebbels S; Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.
  • Kaeser PS; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  • Kay JN; Department of Neurobiology and Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA.
  • Lu W; Synapse and Neural Circuit Research Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
  • Luo L; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Mandai K; Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan; Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagam
  • McBain CJ; Section on Cellular and Synaptic Physiology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
  • Nave KA; Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.
  • Prado MAM; Robarts Research Institute, Department of Anatomy and Cell Biology, and Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON N6A 5B7, Canada; Brain and Mind Institute, The University of Western Ontario, London, ON N6A 5B7,
  • Prado VF; Robarts Research Institute, Department of Anatomy and Cell Biology, and Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON N6A 5B7, Canada; Brain and Mind Institute, The University of Western Ontario, London, ON N6A 5B7,
  • Rothstein J; Department of Neurology and Brain Science Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Rubenstein JLR; Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, UCSF Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA 94158, USA; Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Saher G; Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany.
  • Sakimura K; Department of Cellular Neurobiology, Brain Research Institute, Niigata University, Niigata 951-8585, Japan.
  • Sanes JR; Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
  • Scheiffele P; Biozentrum of the University of Basel, Basel, Switzerland.
  • Takai Y; Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 1-5-6 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
Neuron ; 106(1): 37-65.e5, 2020 04 08.
Article em En | MEDLINE | ID: mdl-32027825
ABSTRACT
The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines designed for nervous system-specific recombination. Selective maternal or paternal germline recombination is showcased with sample Cre lines. Collated data reveal germline recombination in over half of 64 commonly used Cre driver lines, in most cases with a parental sex bias related to Cre expression in sperm or oocytes. Slight differences among Cre driver lines utilizing common transcriptional control elements affect germline recombination rates. Specific target loci demonstrated differential recombination; thus, reporters are not reliable proxies for another locus of interest. Similar principles apply to other recombinase systems and other genetically targeted organisms. We hereby draw attention to the prevalence of germline recombination and provide guidelines to inform future research for the neuroscience and broader molecular genetics communities.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Recombinação Genética / Espermatozoides / Marcação de Genes / Integrases / Neurônios Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Recombinação Genética / Espermatozoides / Marcação de Genes / Integrases / Neurônios Tipo de estudo: Prevalence_studies / Risk_factors_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article