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Improved cancer inhibition by miR-143 with a longer passenger strand than natural miR-143.
Ida, Hiroyuki; Tanabe, Toshizumi; Tachibana, Akira.
Afiliação
  • Ida H; Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka, 558-8585, Japan.
  • Tanabe T; Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka, 558-8585, Japan.
  • Tachibana A; Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka, 558-8585, Japan. Electronic address: akira-tachibana@osaka-cu.ac.jp.
Biochem Biophys Res Commun ; 524(4): 810-815, 2020 04 16.
Article em En | MEDLINE | ID: mdl-32037092
We improved miR-143, which inhibits the growth of cancer cells, by the replacement of the passenger strand. As a result, new miR-143 variants were developed with a single mismatch at the 4th position from the 3'-terminal of the guide strand and an RNA passenger strand with a G-rich flanking DNA region. A reporter gene assay showed that the 80% inhibitory concentration of the new miR-143, long miR-143, was 69 pM, which was three times lower than that of natural miR-143. Long miR-143 inhibited the growth of two cancer cell lines, HeLa-S3 and MIAPaCa-2, more effectively than natural miR-143. This method could be applied to other miRNA families and should be useful for the development of miRNA drugs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligonucleotídeos / RNA Mensageiro / Regulação Neoplásica da Expressão Gênica / Inativação Gênica / MicroRNAs Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligonucleotídeos / RNA Mensageiro / Regulação Neoplásica da Expressão Gênica / Inativação Gênica / MicroRNAs Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article