Rapid Identification Method for Gamma-Irradiated Soybeans Using Gas Chromatography-Mass Spectrometry Coupled with a Headspace Solid-Phase Microextraction Technique.
J Agric Food Chem
; 68(9): 2803-2815, 2020 Mar 04.
Article
em En
| MEDLINE
| ID: mdl-32037818
ABSTRACT
This study evaluated the applicability of a rapid analytical method using a headspace solid-phase microextraction gas chromatography/mass spectrometry (HS-SPME-GC/MS) technique to identify gamma-irradiated soybeans (0.1-5 kGy). From the partial least squares discriminant analysis used to discriminate between non-irradiated and irradiated soybean samples, 1,7-hexadecadiene was selected as the identifying marker. Response surface methodology experiments were used to determine the optimal HS-SPME extraction conditions including a carboxen/polydimethylsiloxane fiber with an extraction temperature of 98 °C and an extraction time of 55 min. 1,7-Hexdecadiene was detected in all samples irradiated at ≥ 0.1 kGy under the optimized HS-SPME-GC/MS conditions, and the unique presence of the marker in a gamma-irradiated sample was verified by comparing the results from heat, steam, microwave, sonication, and ultraviolet treatments. The comparisons of the identification properties for various conventional methods validated several advances in HS-SPME-GC/MS analysis in terms of rapid analysis, high sensitivity, and absence of solvent.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glycine max
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Extratos Vegetais
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Microextração em Fase Sólida
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Cromatografia Gasosa-Espectrometria de Massas
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article