ß-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines.
J Res Med Sci
; 25: 7, 2020.
Article
em En
| MEDLINE
| ID: mdl-32055247
ABSTRACT
BACKGROUND:
Cholangiocarcinoma (CCA) is a neglected disease prevalent in developing countries with high burden and mortality rate, and there is no effective treatment. We aimed to investigate ß-eudesmol molecular target of action in human CCA cell lines using the selected key molecules of apoptotic pathways. MATERIALS ANDMETHODS:
Two CCA cell lines (HuH28 and HuCCT1) were assessed at different time points after ß-eudesmol treatment for mRNA and protein expression profiles of caspase-3, -8, -9, p53, p21, Bcl-2, and Bax by real-time polymerase chain reaction and western blot, respectively.RESULTS:
ß-eudesmol induced expressions of p21 and p53 in mRNA/protein level in HuH28 and HuCCT1 cells. These CCA cells also expressed caspase-3, -8, -9 and bax (mRNA and/or protein level) among others after ß-eudesmol treatment indicating its role in both intrinsic and extrinsic caspase-dependent apoptotic pathways.CONCLUSION:
The study demonstrated that ß-eudesmol induced the expression of apoptosis pathway proteins, suggesting its potential role in promoting the caspase-dependent apoptotic pathway, and induction of the cell cycle arrest in CCA cell lines. ß-eudesmol can be considered as a potential compound for further investigation as an anti-CCA agent.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article