Phylogenomic assessment of drug-resistant Mycobacterium tuberculosis strains from Beira, Mozambique.
Tuberculosis (Edinb)
; 121: 101905, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-32063558
ABSTRACT
BACKGROUND:
Mozambique is a high-burden tuberculosis (TB) country where TB/HIV co-infection and drug resistant TB (DR-TB) incidence is increasing. Whole genome sequencing (WGS) comprehensively describes the molecular epidemiology of TB, allows prediction of DR-TB phenotypes, lineages strains identification and better understanding of transmission chains.OBJECTIVE:
To describe genetic diversity of DR-TB Mycobacterium tuberculosis isolated in Beira, Mozambique.METHODS:
Descriptive cross-sectional study with 35 M. tuberculosis isolates, resistant to at least one first-line drug on molecular drug-susceptibility tests (DST). Variant identification, DR prediction and phylogenetic analysis provided by WGS, drug-susceptibility pattern compared to line-probe assay (LPA) Genotype MTBDRTMplus and MTBDRTMsl.FINDINGS:
Lineage 4 (L4) was the most prevalent 25 (71.4%) isolates; 5 (14.3%) L1 and 5 (14.3%) L2. WGS showed 33/35 (94.3%) isolates resistant to at least one drug, two pan-susceptible isolates that were previously diagnosed as DR-TB with genotype MTBDRplus. Concordance between WGS and LPA 88.6% for isoniazid (INH), 85.7% to rifampicin (RPM), 91.4% for quinolones and 100% to second line injectable drugs. There were three possible TB transmission chains, 10 strains showing recent transmission.CONCLUSION:
WGS provided reliable information about the most frequent lineages related to DR-TB in Beira, Mozambique L4.3 (LAM), L2 (Beijing) and L1 (EAI) and possible recent transmission chain.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tuberculose Resistente a Múltiplos Medicamentos
/
Farmacorresistência Bacteriana Múltipla
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Mycobacterium tuberculosis
Tipo de estudo:
Observational_studies
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Prevalence_studies
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Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
País/Região como assunto:
Africa
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article