Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium.
In Vitro Cell Dev Biol
; 24(12): 1223-8, 1988 Dec.
Article
em En
| MEDLINE
| ID: mdl-3209588
ABSTRACT
The proximate cholesterol precursors lathosterol, 7-dehydrocholesterol and desmosterol supported the growth of NS-1 and X63 mouse myeloma cells. These cells and X63.653 cells are cholesterol auxotrophs, yet each was able to convert [3H]lathosterol to [3H]cholesterol. These results are consistent with the conclusion that cholesterol auxotrophy in these myeloma cells is due to a deficiency in 3-ketosteroid reductase activity. The steroid hormones testosterone, progesterone and hydrocortisone could not replace cholesterol as a medium supplement. These results provide a greater understanding of the cholesterol auxotrophy characteristic of cell lines clonally-derived from the MOPC 21 myeloma tumor, and they provide a rational basis for the use of sterols in defined culture medium for mouse myeloma cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colestadienóis
/
Colesterol
/
Desidrocolesteróis
/
Desmosterol
/
Hormônios
/
Mieloma Múltiplo
Limite:
Animals
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article