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Stress Reshapes the Physiological Response of Halophile Fungi to Salinity.
Pérez-Llano, Yordanis; Rodríguez-Pupo, Eya Caridad; Druzhinina, Irina S; Chenthamara, Komal; Cai, Feng; Gunde-Cimerman, Nina; Zalar, Polona; Gostincar, Cene; Kostanjsek, Rok; Folch-Mallol, Jorge Luis; Batista-García, Ramón Alberto; Sánchez-Carbente, María Del Rayo.
Afiliação
  • Pérez-Llano Y; Center of Research on Cell Dynamics, Autonomous University of the State of Morelos, Morelos 62210, Mexico.
  • Rodríguez-Pupo EC; Center of Research on Cell Dynamics, Autonomous University of the State of Morelos, Morelos 62210, Mexico.
  • Druzhinina IS; Institute of Chemical, Environmental and Bioscience Engineering (ICEBE), TU Wien, 1060 Vienna, Austria.
  • Chenthamara K; Fungal Genomics Group, Nanjing Agricultural University, Nanjing, 210095, China.
  • Cai F; Institute of Chemical, Environmental and Bioscience Engineering (ICEBE), TU Wien, 1060 Vienna, Austria.
  • Gunde-Cimerman N; Institute of Chemical, Environmental and Bioscience Engineering (ICEBE), TU Wien, 1060 Vienna, Austria.
  • Zalar P; Fungal Genomics Group, Nanjing Agricultural University, Nanjing, 210095, China.
  • Gostincar C; Department of Biology, Biotechnical Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
  • Kostanjsek R; Department of Biology, Biotechnical Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
  • Folch-Mallol JL; Department of Biology, Biotechnical Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
  • Batista-García RA; Department of Biology, Biotechnical Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
  • Sánchez-Carbente MDR; Laboratory of Molecular Biology of Fungi, Center for Research on Biotechnology, Autonomous University of the State of Morelos, Morelos 62210, Mexico.
Cells ; 9(3)2020 02 25.
Article em En | MEDLINE | ID: mdl-32106416
ABSTRACT
(1)

Background:

Mechanisms of cellular and molecular adaptation of fungi to salinity have been commonly drawn from halotolerant strains and few studies in basidiomycete fungi. These studies have been conducted in settings where cells are subjected to stress, either hypo- or hyperosmotic, which can be a confounding factor in describing physiological mechanisms related to salinity. (2)

Methods:

We have studied transcriptomic changes in Aspergillussydowii, a halophilic species, when growing in three different salinity conditions (No NaCl, 0.5 M, and 2.0 M NaCl). (3)

Results:

In this fungus, major physiological modifications occur under high salinity (2.0 M NaCl) and not when cultured under optimal conditions (0.5 M NaCl), suggesting that most of the mechanisms described for halophilic growth are a consequence of saline stress response and not an adaptation to saline conditions. Cell wall modifications occur exclusively at extreme salinity, with an increase in cell wall thickness and lamellar structure, which seem to involve a decrease in chitin content and an augmented content of alfa and beta-glucans. Additionally, three hydrophobin genes were differentially expressed under hypo- or hyperosmotic stress but not when the fungus grows optimally. Regarding compatible solutes, glycerol is the main compound accumulated in salt stress conditions, whereas trehalose is accumulated in the absence of salt. (4)

Conclusions:

Physiological responses to salinity vary greatly between optimal and high salt concentrations and are not a simple graded effect as the salt concentration increases. Our results highlight the influence of stress in reshaping the response of extremophiles to environmental challenges.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estresse Fisiológico / Fungos Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estresse Fisiológico / Fungos Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article