Rapid Detection of Nocardia by Next-Generation Sequencing.
Front Cell Infect Microbiol
; 10: 13, 2020.
Article
em En
| MEDLINE
| ID: mdl-32133300
ABSTRACT
In this original study, we retrospectively reviewed the cases of nocardiosis diagnosed through culture and next-generation sequencing (NGS) methods between 2014 and 2018 in Huashan Hospital and found out that the latter way can not only improve the detection rate of Nocardia spp. but also greatly reduce the turnaround time. In addition, by comparing nocardiosis and non-nocardiosis patients both of whose samples had Nocardia spp. detected by NGS, we found that Nocardia's specific reads ranking among top two might be a satisfactory cutoff value for clinical diagnosis of the disease. Our study introduced the promising value of the NGS method in the rapid diagnosis of nocardiosis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de DNA
/
Sequenciamento de Nucleotídeos em Larga Escala
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Nocardia
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Nocardiose
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Female
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Humans
/
Male
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article