Improvement of the dP-nucleoside-mediated herpes simplex virus thymidine kinase negative-selection system by manipulating dP metabolism genes.

Kawai-Noma, Shigeko; Saeki, Kazuya; Yumoto, Tatsuya; Minakata, Katsuya; Saito, Kyoichi; Umeno, Daisuke

Kawai-Noma, Shigeko; Saeki, Kazuya; Yumoto, Tatsuya; Minakata, Katsuya; Saito, Kyoichi; Umeno, Daisuke.

Afiliação

Kawai-Noma S; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba 263-8522, Japan. Electronic address: kawai-noma@chiba-u.jp.
Saeki K; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba 263-8522, Japan.
Yumoto T; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba 263-8522, Japan.
Minakata K; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba 263-8522, Japan.
Saito K; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba 263-8522, Japan.
Umeno D; Department of Applied Chemistry and Biotechnology, Chiba University, Chiba 263-8522, Japan.

J Biosci Bioeng ; 130(2): 121-127, 2020 Aug.

Article em En | MEDLINE | ID: mdl-32229092

Assuntos

Engenharia Genética/métodos; Nucleosídeos/metabolismo; Simplexvirus; Timidina Quinase/genética; Timidina Quinase/metabolismo; Desoxirribonucleosídeos/farmacologia; Escherichia coli/genética; Proteínas de Escherichia coli/genética; Mutagênese/efeitos dos fármacos; Simplexvirus/enzimologia; Simplexvirus/genética

Palavras-chave

Escherichia coli; Genome engineering; Herpes simplex virus thymidine kinase; Mutagenic nucleoside analog 6-(ß-d-2-deoxyribofuranosyl)-3,4-dihydro-8H-pyrimido [4,5-c][1,2] oxazin-7-one; Negative selection system

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Timidina Quinase / Engenharia Genética / Simplexvirus / Nucleosídeos Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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