Characterization and effect of nanocomplexed fluoride solutions on the inhibition of enamel demineralization created by a multispecies cariogenic biofilm model.
Clin Oral Investig
; 24(11): 3947-3959, 2020 Nov.
Article
em En
| MEDLINE
| ID: mdl-32236723
ABSTRACT
OBJECTIVES:
The aim of this study was to assess the in vitro caries preventive effect of nanocomplexed solutions of hydroxypropyl-ß-cyclodextrin and γ-cyclodextrin associated with titanium tetrafluoride (TiF4) after different complexation times (12 or 72 h). MATERIALS ANDMETHODS:
Enamel blocks were randomly distributed in 9 groups (n = 11) negative control, hydroxypropyl-ß-cyclodextrin, γ-cyclodextrin, TiF4, hydroxypropyl-ß-cyclodextrinTiF4 12 h, hydroxypropyl-ß-cyclodextrinTiF4 72 h, γ-cyclodextrinTiF4 12 h, γ-cyclodextrinTiF4 72 h, and NaF (positive control). The solutions were applied for 1 min and the blocks were exposed to a biofilm model. Nanocompounds were characterized by differential scanning calorimetry and X-ray powder diffraction. The percentage of surface microhardness loss (%SML), mineral density changes (ΔZ), lesion depth, surface morphology (scanning electron microscopy-SEM), and chemical characterization (energy-dispersive spectroscopy-EDS) were assessed.RESULTS:
No oxidation was observed, and the formation of the nanocomplexes was evidenced by changes in the melting point compared to pure cyclodextrins and the loss of crystallinity of the materials. Hydroxypropyl-ß-cyclodextrinTiF4 72 h resulted in lower %SML than negative control, hydroxypropyl-ß-cyclodextrin, γ-cyclodextrin, and TiF4 (p < 0.05). NaF differed from all groups (p < 0.05), except for hydroxypropyl-ß-cyclodextrinTiF4 72 h (p = 0.83). ΔZ of hydroxypropyl-ß-cyclodextrinTiF4 72 h was higher than negative control, hydroxypropyl-ß-cyclodextrin, γ-cyclodextrin, γ-cyclodextrinTiF4 1 2 h, γ-cyclodextrinTiF4 72 h, and NaF (p < 0.05) and similar to TiF4 and hydroxypropyl-ß-cyclodextrinTiF4 12 h (p > 0.05). SEM/EDS detected Ti in the blocks subjected to TiF4-products.CONCLUSION:
The hydroxypropyl-ß-cyclodextrinTiF4 72 h solution showed caries preventive effect on the surface and subsurface of the enamel. CLINICAL RELEVANCE A hydroxypropyl-ß-cyclodextrin nanosystem, in association with TiF4 after 72 h of complexation, may be a promising agent for the prevention of enamel demineralization.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fluoreto de Sódio
/
Fluoretos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article