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JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice.
Liu, Yue; Fan, Jiaying; Yan, Yan; Dang, Xuening; Zhao, Ran; Xu, Yimei; Ding, Zhide.
Afiliação
  • Liu Y; Department of Histology, Embryology, Genetics and Developmental Biology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University School of Medicine, China.
  • Fan J; Department of Histology, Embryology, Genetics and Developmental Biology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University School of Medicine, China.
  • Yan Y; Center for Experimental Medical Science Education, Shanghai Jiao Tong University School of Medicine, China.
  • Dang X; Department of Clinical Medicine, Shanghai Jiao Tong University School of Medicine, China.
  • Zhao R; Department of Clinical Medicine, Shanghai Jiao Tong University School of Medicine, China.
  • Xu Y; Department of Clinical Medicine, Shanghai Jiao Tong University School of Medicine, China.
  • Ding Z; Department of Clinical Medicine, Shanghai Jiao Tong University School of Medicine, China.
FEBS J ; 287(24): 5478-5497, 2020 12.
Article em En | MEDLINE | ID: mdl-32279424
Sertoli cells are crucial for spermatogenesis in the seminiferous epithelium because their actin cytoskeleton supports vesicular transport, cell junction formation, protein anchoring, and spermiation. Here, we show that a junction-mediating and actin-regulatory protein (JMY) affects the blood-tissue barrier (BTB) function through remodeling of the Sertoli cell junctional integrity and it also contributes to controlling endocytic vesicle trafficking. These functions are critical for the maintenance of sperm fertility since loss of Sertoli cell-specific Jmy function induced male subfertility in mice. Specifically, these mice have (a) impaired BTB integrity and spermatid adhesion in the seminiferous tubules; (b) high incidence of sperm structural deformity; and (c) reduced sperm count and poor sperm motility. Moreover, the cytoskeletal integrity was compromised along with endocytic vesicular trafficking. These effects impaired junctional protein recycling and reduced Sertoli cell BTB junctional integrity. In addition, JMY interaction with actin-binding protein candidates α-actinin1 and sorbin and SH3 domain containing protein 2 was related to JMY activity, and in turn, actin cytoskeletal organization. In summary, JMY affects the control of spermatogenesis through the regulation of actin filament organization and endocytic vesicle trafficking in Sertoli cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Sertoli / Espermatogênese / Espermatozoides / Transativadores / Proteínas de Ciclo Celular / Proteoma / Infertilidade Masculina Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células de Sertoli / Espermatogênese / Espermatozoides / Transativadores / Proteínas de Ciclo Celular / Proteoma / Infertilidade Masculina Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article