An adaptable analysis workflow for characterization of platelet spreading and morphology.
Platelets
; 32(1): 54-58, 2021 Jan 02.
Article
em En
| MEDLINE
| ID: mdl-32321340
ABSTRACT
The assessment of platelet spreading through light microscopy, and the subsequent quantification of parameters such as surface area and circularity, is a key assay for many platelet biologists. Here we present an analysis workflow which robustly segments individual platelets to facilitate the analysis of large numbers of cells while minimizing user bias. Image segmentation is performed by interactive learning and touching platelets are separated with an efficient semi-automated protocol. We also use machine learning methods to robustly automate the classification of platelets into different subtypes. These adaptable and reproducible workflows are made freely available and are implemented using the open-source software KNIME and ilastik.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Processamento de Imagem Assistida por Computador
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article