Organellar Maps Through Proteomic Profiling - A Conceptual Guide.
Mol Cell Proteomics
; 19(7): 1076-1087, 2020 07.
Article
em En
| MEDLINE
| ID: mdl-32345598
ABSTRACT
Protein subcellular localization is an essential and highly regulated determinant of protein function. Major advances in mass spectrometry and imaging have allowed the development of powerful spatial proteomics approaches for determining protein localization at the whole cell scale. Here, a brief overview of current methods is presented, followed by a detailed discussion of organellar mapping through proteomic profiling. This relatively simple yet flexible approach is rapidly gaining popularity, because of its ability to capture the localizations of thousands of proteins in a single experiment. It can be used to generate high-resolution cell maps, and as a tool for monitoring protein localization dynamics. This review highlights the strengths and limitations of the approach and provides guidance to designing and interpreting profiling experiments.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Frações Subcelulares
/
Organelas
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Fracionamento Celular
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Proteoma
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Mapeamento de Interação de Proteínas
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Proteômica
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article