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Characterization of a major quantitative trait locus on the short arm of chromosome 4B for spike number per unit area in common wheat (Triticum aestivum L.).
Li, Jinghui; Wen, Shaozhe; Fan, Chaofeng; Zhang, Minghu; Tian, Shuai; Kang, Wenjing; Zhao, Wenxin; Bi, Chan; Wang, Qiuyan; Lu, Shuang; Guo, Weilong; Ni, Zhongfu; Xie, Chaojie; Sun, Qixin; You, Mingshan.
Afiliação
  • Li J; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Wen S; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Fan C; Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
  • Zhang M; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Tian S; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Kang W; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Zhao W; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Bi C; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Wang Q; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Lu S; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Guo W; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Ni Z; National Plant Gene Research Centre, Beijing, 100193, China.
  • Xie C; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
  • Sun Q; National Plant Gene Research Centre, Beijing, 100193, China.
  • You M; State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193,, China.
Theor Appl Genet ; 133(7): 2259-2269, 2020 Jul.
Article em En | MEDLINE | ID: mdl-32347319
ABSTRACT
KEY MESSAGE An InDel marker closely linked with a major and stable quantitative trait locus (QTL) on chromosome 4BS, QSnpa.cau-4B, controlling spike number per unit area will benefit wheat yield improvement. Spike number per unit area (SNPA) is an essential yield-related trait, and analyzing its genetic basis is important for cultivar improvement in wheat (Triticum aestivum L.). In this study, we used the F2 population derived from a cross between two wheat accessions displaying significant differences in SNPA to perform quantitative trait locus (QTL) analysis. Through bulked segregant analysis, a major and stable QTL that explained 18.11-82.11% of the phenotypic variation was identified on chromosome 4BS. The QTL interval was validated using F45 and F67 families and narrowed it to a 24.91-38.36 Mb region of chromosome 4BS according to the 'Chinese Spring' reference genome sequence. In this region, variations in 16 genes caused amino acid changes and three genes were present in only one parent. Among these, we annotated a gene orthologous to TB1 in maize (Zea mays), namely TraesCS4B01G042700, which carried a 44-bp deletion in its promoter in the higher-SNPA parent. An InDel marker based on the insertion/deletion polymorphism was designed and used to diagnose the allelic distribution within a natural population. The frequency of the 44-bp deletion allele associated with higher SNPA was relatively low (13.24%), implying that this favorable allele has not been widely utilized and could be valuable for wheat yield improvement. In summary, we identified a major and stable QTL for SNPA and developed a diagnostic marker for the more-spiked trait, which will be beneficial for molecular-assisted breeding in wheat.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triticum / Genes de Plantas / Cromossomos de Plantas / Locos de Características Quantitativas Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triticum / Genes de Plantas / Cromossomos de Plantas / Locos de Características Quantitativas Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article