Three New Diketopiperazines from the Previously Uncultivable Marine Bacterium Gallaecimonas mangrovi HK-28 Cultivated by iChip.
Chem Biodivers
; 17(7): e2000221, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32347603
ABSTRACT
The inâ
situ application of iChip cultivation in mangrove sediment from Hainan province, China, led to the isolation of a novel bacterial species Gallaecimonas mangrovi HK-28. The extract of G. mangrovi HK-28 exhibited antibiotic activity against the aquatic pathogen Vibrio harveyi, and its chemical constituents were further investigated by bioactivity-guided isolation. Three new diketopiperazines, gallaecimonamides A-C, were accordingly isolated from the AcOEt extract of the fermentation broth of G. mangrovi HK-28. The planar structures of gallaecimonamides A-C were determined using HR-ESI-MS together with 1D- and 2D-NMR. The absolute configurations of gallaecimonamides A-C were assigned by optical rotation, NOESY experiment and TDDFT ECD calculations. The inâ
vitro antibacterial and antimalarial activities of gallaecimonamides A-C were assessed. Gallaecimonamide A was found to display antibacterial activity against V. harveyi with a MIC value of 50â
µm. However, gallaecimonamides B and C showed no antibacterial activity against V. harveyi (MIC >300â
µm). In addition, all the isolates did not exhibit any inhibitory activities against V. parahaemolyticus (MIC>300â
µm) and Plasmodium falciparum W2 (EC50 >100â
µg/mL).
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vibrio
/
Gammaproteobacteria
/
Dicetopiperazinas
/
Antibacterianos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article