Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library.
Biochem Biophys Res Commun
; 533(2): 215-222, 2020 12 03.
Article
em En
| MEDLINE
| ID: mdl-32359876
ABSTRACT
DNA-encoded chemical library (DEL) has emerged as a powerful technology for ligand discovery in biomedical research. Recently, we have developed a DNA-encoded dynamic library (DEDL) approach by incorporating the concept of dynamic combinatorial library (DCL) with DELs. DEDL has shown excellent potential in ligand discovery towards a variety of protein targets. However, the requirement of having a pair of unnatural p-stilbazoles as the interstrand DNA crosslinker has limited the chemical diversity of DEDLs. Here, we replaced p-stilbazole with psoralen (PS) and tested the feasibility of psoralen as the crosslinker in DEDL selection. Since psoralen is commercially available and does not require any special crosslinking partner, existing DELs may be directly used to create high-diversity DEDLs. This study is expected to greatly facilitate the development of DEDLs as a versatile tool in drug discovery.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
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Reagentes de Ligações Cruzadas
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Bibliotecas de Moléculas Pequenas
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Ficusina
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article