Microtubules control cellular shape and coherence in amoeboid migrating cells.
J Cell Biol
; 219(6)2020 06 01.
Article
em En
| MEDLINE
| ID: mdl-32379884
ABSTRACT
Cells navigating through complex tissues face a fundamental challenge while multiple protrusions explore different paths, the cell needs to avoid entanglement. How a cell surveys and then corrects its own shape is poorly understood. Here, we demonstrate that spatially distinct microtubule dynamics regulate amoeboid cell migration by locally promoting the retraction of protrusions. In migrating dendritic cells, local microtubule depolymerization within protrusions remote from the microtubule organizing center triggers actomyosin contractility controlled by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin localization, thereby causing two effects that rate-limit locomotion (1) impaired cell edge coordination during path finding and (2) defective adhesion resolution. Compromised shape control is particularly hindering in geometrically complex microenvironments, where it leads to entanglement and ultimately fragmentation of the cell body. We thus demonstrate that microtubules can act as a proprioceptive device they sense cell shape and control actomyosin retraction to sustain cellular coherence.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
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Actomiosina
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Movimento Celular
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Proteína rhoA de Ligação ao GTP
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Centro Organizador dos Microtúbulos
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Fatores de Troca de Nucleotídeo Guanina Rho
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Microtúbulos
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article