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Key role of the macrophage microtubule network in the intracellular lifestyle of Leishmania amazonensis.
Cojean, Sandrine; Nicolas, Valérie; Lievin-Le Moal, Vanessa.
Afiliação
  • Cojean S; CNRS, UMR 8076 BioCis, University Paris-Saclay, Châtenay-Malabry, France.
  • Nicolas V; Institut Paris-Saclay d'Innovation Thérapeutique (IPSIT), UMS -US31 -UMS3679, Microscopy facility (MIPSIT), University Paris-Saclay, Châtenay-Malabry, France.
  • Lievin-Le Moal V; Inserm, UMR-S 996 Inflammation, Microbiome and Immunosurveillance, University Paris-Saclay, Clamart, France.
Cell Microbiol ; 22(9): e13218, 2020 09.
Article em En | MEDLINE | ID: mdl-32406568
We conducted a study to decipher the mechanism of the formation of the large communal Leishmania amazonensis-containing parasitophorous vacuole (PV) and found that the macrophage microtubule (MT) network dynamically orchestrates the intracellular lifestyle of this intracellular parasite. Physical disassembly of the MT network of macrophage-like RAW 264.7 cells or silencing of the dynein gene, encoding the MT-associated molecular motor that powers MT-dependent vacuolar movement, by siRNA resulted in most of the infected cells hosting only tight parasite-containing phagosome-like vacuoles randomly distributed throughout the cytoplasm, each insulating a single parasite. Only a minority of the infected cells hosted both isolated parasite-containing phagosome-like vacuoles and a small communal PV, insulating a maximum of two to three parasites. The tight parasite-containing phagosome-like vacuoles never matured, whereas the small PVs only matured to a small degree, shown by the absence or faint acquisition of host-cell endolysosomal characteristics. As a consequence, the parasites were unable to successfully complete promastigote-to-amastigote differentiation and died, regardless of the type of insulation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vacúolos / Leishmania mexicana / Macrófagos / Microtúbulos Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vacúolos / Leishmania mexicana / Macrófagos / Microtúbulos Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article