Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro.
Cell Mol Biol (Noisy-le-grand)
; 66(2): 165-171, 2020 May 16.
Article
em En
| MEDLINE
| ID: mdl-32415944
ABSTRACT
The aim of this study was to investigate the effect of three-dimensional (3D) bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells (HUMSCs) on cell viability, proliferation and differentiation in vitro. Functional 3D bio-printed microspheres consisting of HUMSCs were constructed using electrostatic inkjet technique. The parameters used for the synthesis of 3D bio-printed tissue constructs were first optimized. The viability, proliferation and differentiation of 3D cultured HUMSCs were assessed. The results of scanning electron microscopy (SEM) showed that isolated HUMSCs exhibited fibroblast-like spindle adherent growth. The optimized printing parameters were 6 kV voltage, 10 mL/h flow, 15 cm receiving height, and alginate water ratio of 11 mixed at 37 °C. Compared with 2D cultured HUMSCs, the 3D cultured HUMSCs have better viability, proliferation and differentiation ability. The results obtained in this study indicate that 3D bio-printed tissue constructs promote HUMSC viability, proliferation, and neural differentiation in vitro.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cordão Umbilical
/
Diferenciação Celular
/
Células-Tronco Mesenquimais
/
Bioimpressão
/
Impressão Tridimensional
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article