Abolishing spontaneous epileptiform activity in human brain tissue through AMPA receptor inhibition.
Ann Clin Transl Neurol
; 7(6): 883-890, 2020 06.
Article
em En
| MEDLINE
| ID: mdl-32426918
ABSTRACT
OBJECTIVE:
The amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) is increasingly recognized as a therapeutic target in drug-refractory pediatric epilepsy. Perampanel (PER) is a non-competitive AMPAR antagonist, and pre-clinical studies have shown the AMPAR-mediated anticonvulsant effects of decanoic acid (DEC), a major medium-chain fatty acid provided in the medium-chain triglyceride ketogenic diet.METHODS:
Using brain tissue resected from children with intractable epilepsy, we recorded the effects of PER and DEC in vitro.RESULTS:
We found resected pediatric epilepsy tissue exhibits spontaneous epileptic activity in vitro, and showed that DEC and PER inhibit this epileptiform activity in local field potential recordings as well as excitatory synaptic transmission.INTERPRETATION:
This study confirms AMPAR antagonists inhibit epileptiform discharges in brain tissue resected in a wide range of pediatric epilepsies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piridonas
/
Receptores de AMPA
/
Ácidos Decanoicos
/
Epilepsia
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Potenciais Sinápticos
/
Anticonvulsivantes
Limite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article