Genetically encoded intrabody sensors report the interaction and trafficking of ß-arrestin 1 upon activation of G-protein-coupled receptors.

Baidya, Mithu; Kumari, Punita; Dwivedi-Agnihotri, Hemlata; Pandey, Shubhi; Sokrat, Badr; Sposini, Silvia; Chaturvedi, Madhu; Srivastava, Ashish; Roy, Debarati; Hanyaloglu, Aylin C; Bouvier, Michel; Shukla, Arun K

Baidya, Mithu; Kumari, Punita; Dwivedi-Agnihotri, Hemlata; Pandey, Shubhi; Sokrat, Badr; Sposini, Silvia; Chaturvedi, Madhu; Srivastava, Ashish; Roy, Debarati; Hanyaloglu, Aylin C; Bouvier, Michel; Shukla, Arun K.

Afiliação

Baidya M; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Kumari P; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Dwivedi-Agnihotri H; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Pandey S; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Sokrat B; Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, Quebec, Canada.
Sposini S; Department of Biochemistry and Molecular Medicine, Université de Montréal, Montreal, Quebec, Canada.
Chaturvedi M; Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, United Kingdom.
Srivastava A; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Roy D; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Hanyaloglu AC; Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur, India.
Bouvier M; Institute of Reproductive and Developmental Biology, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, United Kingdom.
Shukla AK; Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, Quebec, Canada.

J Biol Chem ; 295(30): 10153-10167, 2020 07 24.

Article em En | MEDLINE | ID: mdl-32439801

Assuntos

Receptores Acoplados a Proteínas G/metabolismo; beta-Arrestina 1/metabolismo; Células HEK293; Humanos; Fragmentos Fab das Imunoglobulinas/genética; Fragmentos Fab das Imunoglobulinas/metabolismo; Transporte Proteico; Receptores Acoplados a Proteínas G/genética; beta-Arrestina 1/genética

Palavras-chave

G-proteincoupled receptors (GPCRs); biased agonism; bioluminescence resonance energy transfer (BRET); biosensors; cell signaling; cellular signaling; confocal microscopy; intrabody; protein phosphorylation; signal transduction; synthetic antibody; trafficking; ß-arrestins

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores Acoplados a Proteínas G / Beta-Arrestina 1 Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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