Growing pains: addressing the pitfalls of plant extracellular vesicle research.
New Phytol
; 228(5): 1505-1510, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-32506490
ABSTRACT
Extracellular vesicles (EVs) are small, membrane-enclosed compartments that mediate the intercellular transport of proteins and small RNAs. In plants, EVs are thought to play a prominent role in immune responses and are being championed as the long-sought-after mechanism for host-induced gene silencing. However, parallel research on mammalian EVs is raising concerns about potential pitfalls faced by all EV researchers that will need to be addressed in order to convincingly establish that EVs are the primary mediators of small RNA transfer between organisms. Here we discuss these pitfalls in the context of plant EV research, with a focus on experimental approaches required to distinguish bona fide EV cargo from merely co-purifying contaminants.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vesículas Extracelulares
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article