Your browser doesn't support javascript.
loading
Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model.
Meirelles, Rogério José de Azevedo; Lizarte Neto, Fermino Sanches; Cirino, Mucio Luiz de Assis; Novais, Paulo Cezar; Gula, Isabella Stracieri; Silva, Jairo Pinheiro da; Tazzima, Maria de Fátima Galli Sorita; Fazan, Valéria Paula Sassoli; Durand, Marina Toledo; Tirapelli, Daniela Pretti da Cunha; Carvalho, Camila Albuquerque Melo de; Schimming, Bruno César; Molina, Carlos Augusto Fernandes; Tucci Junior, Silvio; Tirapelli, Luis Fernando.
Afiliação
  • Meirelles RJA; PhD, Department of Surgery and Anatomy, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirao Preto-SP, Brazil. Acquisition of data.
  • Lizarte Neto FS; PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Technical procedures.
  • Cirino MLA; Fellow PhD degree, Postgraduate Program in Clinical Surgical, Department of Surgery and Anatomy, Surgical Clinic Program, FMRP-USP, Ribeirao Preto-SP, Brazil. Acquisition of data.
  • Novais PC; PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Postgarduate Program in Structural and Functional Interactions in Rehabiliattion, Universidade de Marília (UNIMAR), Marilia-SP, Brazil. Histopathological examinations.
  • Gula IS; Graduate student, School of Medicine, Universidade de Ribeirão Preto (UNAERP), Brazil. Acquisition of data.
  • Silva JPD; PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Professor, Faculdade de Taquaritinga (FTGA), Brazil. Manuscript preparation.
  • Tazzima MFGS; PhD, Assistant Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Manuscript writing.
  • Fazan VPS; PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Manuscript writing.
  • Durand MT; PhD, UNAERP, Ribeirao Preto-SP, Brazil. Manuscript writing.
  • Tirapelli DPDC; PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Final approval.
  • Carvalho CAM; PhD, Department of Surgery and Anatomy, FMRP-USP, and Professor, Centro Universitário Barão de Mauá, Ribeirao Preto-SP, Brazil. Manuscript writing.
  • Schimming BC; PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Associate Professor, Department of Bioscience, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Critical revision.
  • Molina CAF; PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Final approval.
  • Tucci Junior S; PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Final approval.
  • Tirapelli LF; PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Final approval.
Acta Cir Bras ; 35(3): e202000305, 2020.
Article em En | MEDLINE | ID: mdl-32520081
ABSTRACT

PURPOSE:

To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats.

METHODS:

Twenty-four rats were divided into two experimental groups Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue.

RESULTS:

Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups.

CONCLUSION:

Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pênis / Apoptose / Alcoolismo Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pênis / Apoptose / Alcoolismo Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article