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Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation.
Benoni, Roberto; Culka, Martin; Hudecek, Oldrich; Gahurova, Lenka; Cahová, Hana.
Afiliação
  • Benoni R; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences Flemingovo nam. 2, 16610 Prague 6, Czech Republic.
  • Culka M; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences Flemingovo nam. 2, 16610 Prague 6, Czech Republic.
  • Hudecek O; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences Flemingovo nam. 2, 16610 Prague 6, Czech Republic.
  • Gahurova L; Department of Molecular Biology and Genetics, Faculty of Science, University of South Bohemia, Branisovska 1760, 37005 Ceske Budejovice, Czech Republic.
  • Cahová H; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences Flemingovo nam. 2, 16610 Prague 6, Czech Republic.
ACS Chem Biol ; 15(7): 1765-1772, 2020 07 17.
Article em En | MEDLINE | ID: mdl-32530599
Dinucleoside polyphosphates (NpnNs) were discovered 50 years ago in all cells. They are often called alarmones, even though the molecular target of the alarm has not yet been identified. Recently, we showed that they serve as noncanonical initiating nucleotides (NCINs) and fulfill the role of 5' RNA caps in Escherichia coli. Here, we present molecular insight into their ability to be used as NCINs by T7 RNA polymerase in the initiation phase of transcription. In general, we observed NpnNs to be equally good substrates as canonical nucleotides for T7 RNA polymerase. Surprisingly, the incorporation of ApnGs boosts the production of RNA 10-fold. This behavior is due to the pairing ability of both purine moieties with the -1 and +1 positions of the antisense DNA strand. Molecular dynamic simulations revealed noncanonical pairing of adenosine with the thymine of the DNA.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA / Fosfatos de Dinucleosídeos / Iniciação da Transcrição Genética Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA / Fosfatos de Dinucleosídeos / Iniciação da Transcrição Genética Idioma: En Ano de publicação: 2020 Tipo de documento: Article