Direct production of fatty alcohols from glucose using engineered strains of Yarrowia lipolytica.
Metab Eng Commun
; 10: e00105, 2020 Jun.
Article
em En
| MEDLINE
| ID: mdl-32547923
ABSTRACT
Fatty alcohols are important industrial oleochemicals with broad applications and a growing market. Here, we sought to engineer Yarrowia lipolytica to serve as a renewable source of fatty alcohols (specifically hexadecanol, heptadecanol, octadecanol, and oleyl alcohol) directly from glucose. Through screening four fatty acyl-CoA reductase (FAR) enzyme variants across two engineered background strains, we identified that MhFAR enabled the highest production. Further strain engineering, fed-batch flask cultivation, and extractive fermentation improved the fatty alcohol titer to 1.5â¯g/L. Scale-up of this strain in a 2L bioreactor led to 5.8â¯g/L total fatty alcohols at an average yield of 36â¯mg/g glucose with a maximum productivity of 39â¯mg/L hr. Finally, we utilized this fatty alcohol reductase to generate a customized fatty alcohol, linolenyl alcohol, from α-linolenic acid. Overall, this work demonstrates Y. lipolytica is a robust chassis for diverse fatty alcohol production and highlights the capacity to obtain high titers and yields from a purely minimal media formulation directly from glucose without the need for complex additives.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article