An NMR based phosphodiesterase assay.
Chem Commun (Camb)
; 56(58): 8091-8094, 2020 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-32555789
ABSTRACT
We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diester Fosfórico Hidrolases
/
Ressonância Magnética Nuclear Biomolecular
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Ensaios Enzimáticos
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article