Characterizing the Fused TvG6PD::6PGL Protein from the Protozoan Trichomonas vaginalis, and Effects of the NADP+ Molecule on Enzyme Stability.
Int J Mol Sci
; 21(14)2020 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-32650494
ABSTRACT
This report describes a functional and structural analysis of fused glucose-6-phosphate dehydrogenase dehydrogenase-phosphogluconolactonase protein from the protozoan Trichomonas vaginalis (T. vaginalis). The glucose-6-phosphate dehydrogenase (g6pd) gene from T. vaginalis was isolated by PCR and the sequence of the product showed that is fused with 6pgl gene. The fused Tvg6pd6pgl gene was cloned and overexpressed in a heterologous system. The recombinant protein was purified by affinity chromatography, and the oligomeric state of the TvG6PD6PGL protein was found as tetramer, with an optimal pH of 8.0. The kinetic parameters for the G6PD domain were determined using glucose-6-phosphate (G6P) and nicotinamide adenine dinucleotide phosphate (NADP+) as substrates. Biochemical assays as the effects of temperature, susceptibility to trypsin digestion, and analysis of hydrochloride of guanidine on protein stability in the presence or absence of NADP+ were performed. These results revealed that the protein becomes more stable in the presence of the NADP+. In addition, we determined the dissociation constant for the binding (Kd) of NADP+ in the protein and suggests the possible structural site in the fused TvG6PD6PGL protein. Finally, computational modeling studies were performed to obtain an approximation of the structure of TvG6PD6PGL. The generated model showed differences with the GlG6PD6PGL protein (even more so with human G6PD) despite both being fused.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trichomonas vaginalis
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Estabilidade Enzimática
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Proteínas Recombinantes
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Hidrolases de Éster Carboxílico
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Proteínas de Protozoários
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Glucosefosfato Desidrogenase
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NADP
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article