An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity.
Immunity
; 53(1): 217-232.e5, 2020 07 14.
Article
em En
| MEDLINE
| ID: mdl-32668225
ABSTRACT
B cells are capable of a wide range of effector functions including antibody secretion, antigen presentation, cytokine production, and generation of immunological memory. A consistent strategy for classifying human B cells by using surface molecules is essential to harness this functional diversity for clinical translation. We developed a highly multiplexed screen to quantify the co-expression of 351 surface molecules on millions of human B cells. We identified differentially expressed molecules and aligned their variance with isotype usage, VDJ sequence, metabolic profile, biosynthesis activity, and signaling response. Based on these analyses, we propose a classification scheme to segregate B cells from four lymphoid tissues into twelve unique subsets, including a CD45RB+CD27- early memory population, a class-switched CD39+ tonsil-resident population, and a CD19hiCD11c+ memory population that potently responds to immune activation. This classification framework and underlying datasets provide a resource for further investigations of human B cell identity and function.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Isotipos de Imunoglobulinas
/
Subpopulações de Linfócitos B
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Proteínas de Membrana
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
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Middle aged
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article