Designing enhanced and ratiometric probes for detecting OCl- based on substituents influencing the fluorescence of HBT and their application in strips and bioimaging.
Analyst
; 145(17): 5933-5939, 2020 Aug 24.
Article
em En
| MEDLINE
| ID: mdl-32692325
ABSTRACT
Nitro groups with a strong electron-withdrawing effect can powerfully influence the fluorescence of fluorophores. In this work, through adjusting the nitro group at the HBT fluorophore to construct a phenylhydrazone structure, two probes (HBTN and HBTH) were synthesized to detect OCl-. Consequently, HBTN with the nitro group quenched the fluorescence of HBT and HBTH without the nitro group causing a redshift of the fluorescence, which resulted in enhanced and ratiometric fluorescence signal changes during the detection process. Among them, HBTN shows good ability to selectively detect OCl- in the concentration range of 0-14 µM with the detection limit of 2.06 nM. Based on HBTN, a portable test strip for detecting OCl- was made for the convenient quantification of the OCl- concentration with a spectrophotometer, and exogenous and endogenous OCl- was successfully imaged in cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article