Brothers in Arms: Structure, Assembly and Function of Arenaviridae Nucleoprotein.
Viruses
; 12(7)2020 07 17.
Article
em En
| MEDLINE
| ID: mdl-32708976
ABSTRACT
Arenaviridae is a family of viruses harbouring important emerging pathogens belonging to the Bunyavirales order. Like in other segmented negative strand RNA viruses, the nucleoprotein (NP) is a major actor of the viral life cycle being both (i) the necessary co-factor of the polymerase present in the L protein, and (ii) the last line of defence of the viral genome (vRNA) by physically hiding its presence in the cytoplasm. The NP is also one of the major players interfering with the immune system. Several structural studies of NP have shown that it features two domains a globular RNA binding domain (NP-core) in its N-terminal and an exonuclease domain (ExoN) in its C-terminal. Further studies have observed that significant conformational changes are necessary for RNA encapsidation. In this review we revisited the most recent structural and functional data available on Arenaviridae NP, compared to other Bunyavirales nucleoproteins and explored the structural and functional implications. We review the variety of structural motif extensions involved in NP-NP binding mode. We also evaluate the major functional implications of NP interactome and the role of ExoN, thus making the NP a target of choice for future vaccine and antiviral therapy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arenaviridae
/
Montagem de Vírus
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Proteínas do Nucleocapsídeo
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article