Exogenous proline enhances antioxidant enzyme activities but does not mitigate growth inhibition by selenate stress in tobacco BY-2 cells.
Biosci Biotechnol Biochem
; 84(11): 2281-2292, 2020 Nov.
Article
em En
| MEDLINE
| ID: mdl-32729395
ABSTRACT
Selenium (Se) causes oxidative damage to plants. Proline is accumulated as a compatible solute in plants under stress conditions and mitigates stresses. Selenate at 250 µM increased cell death and inhibited the growth of tobacco BY-2 cells while exogenous proline at 10 mM did not mitigate the inhibition by selenate. Selenate increased accumulation of Se and ROS and activities of antioxidant enzymes but not lipid peroxidation in the BY-2 cells. Proline increased Se accumulation and antioxidant enzyme activities but not either ROS accumulation or lipid peroxidation in the selenate-stressed cells. Glutathione (GSH) rather than ascorbic acid (AsA) mitigated the growth inhibition although both reduced the accumulation of ROS induced by selenate. These results indicate that proline increases both antioxidant enzyme activities and Se accumulation, which overall fails to ameliorate the growth inhibition by selenate and that the growth inhibition is not accounted for only by ROS accumulation. Abbreviations APX ascorbate peroxidase; AsA ascorbic acid; BY-2 Bright Yellow-2; CAT catalase; DAI days after inoculation; DW dry weight; FW fresh weight; GSH glutathione; ROS reactive oxygen species.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
/
Prolina
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Estresse Oxidativo
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Ácido Selênico
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Antioxidantes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article