Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology.
Nat Commun
; 11(1): 3881, 2020 08 04.
Article
em En
| MEDLINE
| ID: mdl-32753572
ABSTRACT
Cells typically respond to chemical or physical perturbations via complex signaling cascades which can simultaneously affect multiple physiological parameters, such as membrane voltage, calcium, pH, and redox potential. Protein-based fluorescent sensors can report many of these parameters, but spectral overlap prevents more than ~4 modalities from being recorded in parallel. Here we introduce the technique, MOSAIC, Multiplexed Optical Sensors in Arrayed Islands of Cells, where patterning of fluorescent sensor-encoding lentiviral vectors with a microarray printer enables parallel recording of multiple modalities. We demonstrate simultaneous recordings from 20 sensors in parallel in human embryonic kidney (HEK293) cells and in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), and we describe responses to metabolic and pharmacological perturbations. Together, these results show that MOSAIC can provide rich multi-modal data on complex physiological responses in multiple cell types.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Miócitos Cardíacos
/
Células-Tronco Pluripotentes Induzidas
/
Imagem Óptica
/
Microscopia de Fluorescência
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article