Quercetin attenuates endocrine and metabolic responses to oxytetracycline in silver catfish (Rhamdia quelen).
Comp Biochem Physiol C Toxicol Pharmacol
; 238: 108864, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-32781297
ABSTRACT
This study aimed to verify whether dietary quercetin protects against the detrimental effects induced by oxytetracycline (OTC) administration in silver catfish (Rhamdia quelen). Fish were divided into different experimental groups that received OTC and/or quercetin, either during 14 or 21â¯days. To determine the endocrine system stress response, we have measured the brain mRNA expression levels of corticotropin-releasing hormone (crh), proopiomelanocortins (pomca and pomcb) and some of the pituitary hormones (growth hormone [gh], somatolactin [sl], and prolactin [prl]). We have also quantified the levels of cortisol as well as some metabolites (glucose, glycogen, lactate, and triglycerides) in the plasma. Moreover, the enzymatic activity of hexokinase, phosphorylase (active GPase), fructose-biphosphatase (FBP), glycerol-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, and glutamate dehydrogenase (GDH) and gill Na+/K+-ATPase were measured. The results demonstrated that OTC activates the silver catfish stress response by increasing the plasma cortisol and decreasing the glucose levels at 14 and 21â¯days. Additionally, OTC also altered the fish hepatic metabolic status as demonstrated by an increase in triglycerides levels and the enzymatic activity of both FBP and GDH after 14â¯days. OTC also stimulated Na+/K+-ATPase activity in the gill after 14â¯days and altered the hypophyseal expression of gh (at 14 and 21â¯days) and prl (at 14â¯days). The co-treatment with 1.5â¯g of quercetin could prevent most of the alterations caused by OTC, strongly suggesting quercetin as a beneficial compound when added to the fish diet.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxitetraciclina
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Hormônios Hipofisários
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Quercetina
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Peixes-Gato
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Sistema Endócrino
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article