Endoplasmic reticulum stress mediates cortical neuron apoptosis after experimental subarachnoid hemorrhage in rats.
Int J Clin Exp Pathol
; 13(7): 1569-1577, 2020.
Article
em En
| MEDLINE
| ID: mdl-32782675
ABSTRACT
OBJECTIVE:
Neuronal apoptosis plays an important pathological process in early brain injury (EBI) after subarachnoid hemorrhage (SAH). This pathological process leads to a poor neurological prognosis for patients. This study aimed to investigate whether endoplasmic reticulum (ER) stress mediates cortical neuron apoptosis in EBI after SAH.METHODS:
Eighty-four male Sprague-Dawley rats were randomly assigned to different groups as follows the control and the 3, 6, 12, 24, 48, and 72 h groups after SAH. The SAH model was established by injecting 0.3 mL of nonheparinized blood into the prechiasmatic cistern. Hematoxylin-eosin staining, Garcia scoring, Western blotting, transmission electron microscopy, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were performed.RESULTS:
SAH reduced the neurological scores and reached a trough at 24 h after the SAH. The GRP78 expression was significantly upregulated at 6 h after the SAH, peaked at 24 h after the SAH, and then decreased. By comparison, the CHOP, caspase-12, ASK1, and p-c-Jun N-terminal kinase expressions were significantly upregulated at 12 h after the SAH and peaked at 24 h after the SAH. The most serious swelling of the rough ER was observed at 24 h after the SAH and remained notably swollen at 72 h after the SAH. The number of TUNEL-positive cells substantially increased significantly at 12 h after the SAH, and the neuronal apoptosis decreased ratio after reaching a peak at 24 h after the SAH. The apoptosis ratio at 72 h after the SAH was still significantly different from the ratio in the control group.CONCLUSION:
Our study clearly demonstrated that ER stress mediates cortical neuron apoptosis after experimental subarachnoid hemorrhage in rats.
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article