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Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays.
Pereira, Adriano E; Huynh, Man P; Sethi, Amit; Miles, Ashley L; Wade French, B; Ellersieck, Mark R; Coudron, Thomas A; Shelby, Kent S; Hibbard, Bruce E.
Afiliação
  • Pereira AE; Division of Plant Sciences, University of Missouri, Columbia, MO.
  • Huynh MP; Division of Plant Sciences, University of Missouri, Columbia, MO.
  • Sethi A; Corteva Agriscience, Johnston, IA.
  • Miles AL; Corteva Agriscience, Johnston, IA.
  • Wade French B; North Central Agricultural Research Laboratory, Brookings, SD.
  • Ellersieck MR; Department of Statistics, University of Missouri, Columbia, MO.
  • Coudron TA; Biological Control Insect Research Laboratory, USDA-ARS, Columbia, MO.
  • Shelby KS; Biological Control Insect Research Laboratory, USDA-ARS, Columbia, MO.
  • Hibbard BE; Plant Genetics Research Unit, USDA/ARS, University of Missouri, Columbia, MO.
J Econ Entomol ; 113(4): 1955-1962, 2020 08 13.
Article em En | MEDLINE | ID: mdl-32789524
The northern corn rootworm (NCR), Diabrotica barberi Smith & Lawrence, is an economic pest of maize in the U.S. Corn Belt. The objective of this study was to determine the baseline susceptibility of a laboratory NCR strain to Bt proteins eCry3.1Ab, mCry3A, Cry3Bb1, and Cry34/35Ab1 using seedling, single plant, and diet-toxicity assays. Plant assays were performed in greenhouse using corn hybrids expressing one of the Bt proteins and each respective near-isoline. Diet-toxicity assays, consisting of Bt proteins overlaid onto artificial diet were also conducted. In both plant assays, significantly more larvae survived Cry34/35Ab1-expressing corn compared with all other Bt-expressing corn, and larvae that survived eCry3.1Ab-expressing corn had significantly smaller head capsule widths compared with larvae that survived Cry34/35Ab1-expressing corn. In seedling assays, larvae surviving eCry3.1Ab-expressing corn also had significantly smaller head capsule widths compared with larvae that survived mCry3A-expressing corn. Additionally, larvae that survived mCry3A-expressing corn weighed significantly more than larvae surviving eCry3.1Ab- and Cry34/35Ab1-expressing corn. In single plant assays, no significant differences in larval dry weight was observed between any of the Bt-expressing corn. In diet assays, LC50s ranged from 0.14 (eCry3.1Ab) to 10.6 µg/cm2 (Cry34/35Ab1), EC50s ranged from 0.12 (Cry34/35Ab1) to 1.57 µg/cm2 (mCry3A), IC50s ranged from 0.08 (eCry3.1Ab) to 2.41 µg/cm2 (Cry34/35Ab1), and MIC50s ranged from 2.52 (eCry3.1Ab) to 14.2 µg/cm2 (mCry3A). These results establish the toxicity of four Bt proteins to a laboratory diapausing NCR strain established prior to the introduction of Bt traits and are important for monitoring resistance evolution in NCR field populations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Besouros Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Besouros Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article