An improved conjugation method for Pseudomonas syringae.
J Microbiol Methods
; 177: 106025, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-32795634
In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Bacteriológicas
/
Conjugação Genética
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Pseudomonas syringae
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article