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An improved conjugation method for Pseudomonas syringae.
Neale, Helen C; Hulin, Michelle T; Harrison, Richard J; Jackson, Robert W; Mansfield, John W; Arnold, Dawn L.
Afiliação
  • Neale HC; Centre for Research in Bioscience, Faculty of Health and Applied Sciences, The University of the West of England, Frenchay Campus, Bristol BS16 1QY, UK.
  • Hulin MT; NIAB EMR, East Malling, UK.
  • Harrison RJ; NIAB EMR, East Malling, UK; NIAB, Cambridge, UK.
  • Jackson RW; Birmingham Institute of Forest Research (BIFoR), University of Birmingham, Birmingham, UK; School of Biosciences, University of Birmingham, Birmingham, UK.
  • Mansfield JW; Faculty of Natural Sciences, Imperial College London, London, UK.
  • Arnold DL; Centre for Research in Bioscience, Faculty of Health and Applied Sciences, The University of the West of England, Frenchay Campus, Bristol BS16 1QY, UK; Harper Adams University, Newport, Shropshire TF10 8NB, UK. Electronic address: darnold@harper-adams.ac.uk.
J Microbiol Methods ; 177: 106025, 2020 10.
Article em En | MEDLINE | ID: mdl-32795634
In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the required increase in frequency and facilitated conjugation of otherwise recalcitrant strains.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Bacteriológicas / Conjugação Genética / Pseudomonas syringae Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Bacteriológicas / Conjugação Genética / Pseudomonas syringae Idioma: En Ano de publicação: 2020 Tipo de documento: Article