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Variability and heritability of mouse brain structure: Microscopic MRI atlases and connectomes for diverse strains.
Wang, Nian; Anderson, Robert J; Ashbrook, David G; Gopalakrishnan, Vivek; Park, Youngser; Priebe, Carey E; Qi, Yi; Laoprasert, Rick; Vogelstein, Joshua T; Williams, Robert W; Johnson, G Allan.
Afiliação
  • Wang N; Duke Center for In Vivo Microscopy, Department of Radiology, Duke University, Duke University Medical Center Box 3302, Durham, NC 27710, USA.
  • Anderson RJ; Duke Center for In Vivo Microscopy, Department of Radiology, Duke University, Duke University Medical Center Box 3302, Durham, NC 27710, USA.
  • Ashbrook DG; Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
  • Gopalakrishnan V; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
  • Park Y; Center for Imaging Science, Johns Hopkins University, Baltimore, MD 21287, USA.
  • Priebe CE; Center for Imaging Science, Johns Hopkins University, Baltimore, MD 21287, USA; Department of Applied Mathematics and Statistics, Johns Hopkins University, Baltimore, MD 21287, USA.
  • Qi Y; Duke Center for In Vivo Microscopy, Department of Radiology, Duke University, Duke University Medical Center Box 3302, Durham, NC 27710, USA.
  • Laoprasert R; Duke Center for In Vivo Microscopy, Department of Radiology, Duke University, Duke University Medical Center Box 3302, Durham, NC 27710, USA.
  • Vogelstein JT; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA; Center for Imaging Science, Johns Hopkins University, Baltimore, MD 21287, USA; Institute for Computational Medicine, Johns Hopkins University, Baltimore, MD 21287, USA; Kavli Neuroscience Discovery Institute,
  • Williams RW; Department of Genetics, Genomics and Informatics, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
  • Johnson GA; Duke Center for In Vivo Microscopy, Department of Radiology, Duke University, Duke University Medical Center Box 3302, Durham, NC 27710, USA; Department of Biomedical Engineering, Duke University, Durham, NC 27710, USA. Electronic address: gjohnson@duke.edu.
Neuroimage ; 222: 117274, 2020 11 15.
Article em En | MEDLINE | ID: mdl-32818613
Genome-wide association studies have demonstrated significant links between human brain structure and common DNA variants. Similar studies with rodents have been challenging because of smaller brain volumes. Using high field MRI (9.4 T) and compressed sensing, we have achieved microscopic resolution and sufficiently high throughput for rodent population studies. We generated whole brain structural MRI and diffusion connectomes for four diverse isogenic lines of mice (C57BL/6J, DBA/2J, CAST/EiJ, and BTBR) at spatial resolution 20,000 times higher than human connectomes. We measured narrow sense heritability (h2) I.e. the fraction of variance explained by strains in a simple ANOVA model for volumes and scalar diffusion metrics, and estimates of residual technical error for 166 regions in each hemisphere and connectivity between the regions. Volumes of discrete brain regions had the highest mean heritability (0.71 ± 0.23 SD, n = 332), followed by fractional anisotropy (0.54 ± 0.26), radial diffusivity (0.34 ± 0.022), and axial diffusivity (0.28 ± 0.19). Connection profiles were statistically different in 280 of 322 nodes across all four strains. Nearly 150 of the connection profiles were statistically different between the C57BL/6J, DBA/2J, and CAST/EiJ lines. Microscopic whole brain MRI/DTI has allowed us to identify significant heritable phenotypes in brain volume, scalar DTI metrics, and quantitative connectomes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Mapeamento Encefálico / Imagem de Tensor de Difusão Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Mapeamento Encefálico / Imagem de Tensor de Difusão Limite: Animals Idioma: En Ano de publicação: 2020 Tipo de documento: Article