Analysis of circular RNA expression profiles of lung cancer in Xuanwei, China.
J Clin Lab Anal
; 34(12): e23521, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-32844469
ABSTRACT
BACKGROUND:
Mounting evidence indicates that circular RNAs (circRNAs) could play a pivotal role in cancers. However, due to the lack of sensitive biomarkers, most lung cancer in Xuanwei (LCXW) patients are still diagnosed at an advanced stage accompany with distant metastasis.METHODS:
According to the stage of LCXW patients and tissue sources, circRNAs microarray detection was carried out in six groups. Considering fold change, raw intensity, the length of circRNAs, and P-value, we selected eightcircRNAs for further study. A total of 50 paired LCXW tissues were carried out real-time quantitative polymerase chain reaction (RT-qPCR) in order to extended sample size to verify the expression of these circRNAs.RESULTS:
We designed 13 617 human circRNA probes for the human circular RNA microarray, detected 10 819 circRNA in six groups of samples; 537 circRNAs were differentially expressed consistently in every stage. Through RT-qPCR, we selected 8 circRNAs, three of which were upregulated (hsa_circ_0005927, hsa_circ_0069397 and hsa_circ_0000937) and five were downregulated (hsa_circ_0001936, hsa_circ_0005255, hsa_circRNA_406010, hsa_circ_0007064, hsa_circ_0000907) in tumor tissues, only hsa_circ_0001936 showed the opposite expression between microarray and RT-qPCR, others were consistent. Additionally, hsa_circ_0005927 and hsa_circ_0001936 were significantly correlated with tumor size, and hsa_circRNA_406010 was related to the prognosis of LCXW patients.CONCLUSION:
Together, these results suggest that hsa_circ_0005927, hsa_circ_0001936, and hsa_circRNA_406010 may serve as the novel potential biomarkers for LCXW. Moreover, these results may provide a new insight for the pathogenesis of LCXW.Palavras-chave
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcriptoma
/
RNA Circular
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Neoplasias Pulmonares
Limite:
Female
/
Humans
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Male
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Middle aged
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article