Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design.

Pryor, John M; Potapov, Vladimir; Kucera, Rebecca B; Bilotti, Katharina; Cantor, Eric J; Lohman, Gregory J S

Pryor, John M; Potapov, Vladimir; Kucera, Rebecca B; Bilotti, Katharina; Cantor, Eric J; Lohman, Gregory J S.

Afiliação

Pryor JM; Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America.
Potapov V; Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America.
Kucera RB; Applications and Product Development, New England Biolabs, Ipswich, Massachusetts, United States of America.
Bilotti K; Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America.
Cantor EJ; Applications and Product Development, New England Biolabs, Ipswich, Massachusetts, United States of America.
Lohman GJS; Research Department, New England Biolabs, Ipswich, Massachusetts, United States of America.

PLoS One ; 15(9): e0238592, 2020.

Article em En | MEDLINE | ID: mdl-32877448

Assuntos

DNA/metabolismo; Biologia Sintética/métodos; DNA Ligases/metabolismo; Enzimas de Restrição do DNA/metabolismo; Nucleotídeos/metabolismo

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Biologia Sintética Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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