High-Dimensional Flow Cytometry Analysis of Regulatory Receptors on Human T Cells, NK Cells, and NKT Cells.
Methods Mol Biol
; 2194: 255-290, 2021.
Article
em En
| MEDLINE
| ID: mdl-32926371
ABSTRACT
The field of flow cytometry has witnessed rapid technological advancements in the last few decades. While the founding principles of fluorescent detection on cells (or particles) within a uniform fluid stream remains largely unchanged, the availability more sensitive cytometers with the ability to multiplex more and more florescent signals has resulted in very complex high-order assays. This results in the co-use of fluorophores with increased levels of emission overlap and/or spillover spreading than in years past and thus requires careful and well thought out planning for flow cytometry assay development. As an example, we present the development of a large 18-color (20 parameter) flow cytometry assay designed to take an in depth analysis of effector lymphocyte phenotypes, with careful attention to assay controls and panel design.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Matadoras Naturais
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Imunofenotipagem
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Células T Matadoras Naturais
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Citometria de Fluxo
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Proteínas de Checkpoint Imunológico
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article