Insecticidal and synergistic activity of dsRNAs targeting buprofezin-specific genes against the small brown planthopper, Laodelphax striatellus.
Arch Insect Biochem Physiol
; 105(4): e21739, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-32929790
ABSTRACT
The small brown planthopper, Laodelphax striatellus, is a dangerous pest in rice fields. Although buprofezin has been used to control L. striatellus for more than a decade, the occurrence of buprofezin-resistant L. striatellus has been recently reported. To develop an alternative pest control strategy, comparative transcriptome analysis of buprofezin-treated and nontreated L. striatellus was performed to screen the buprofezin-specific target genes for RNA interference (RNAi) application. Among six genes downregulated in the buprofezin-treated L. striatellus, RNAi-based silencing of the lipophorin precursor, endocuticle structure glycoprotein, and chitin synthase significantly induced the lethality of L. striatellus in a concentration-dependent manner. In addition, a cocktail of double-stranded RNAs against these three genes showed synergistic effects with buprofezin. These results provide RNAi-based effective approaches to control L. striatellus as well as an efficient method to identify novel target genes for RNAi application.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tiadiazinas
/
Resistência a Inseticidas
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RNA de Cadeia Dupla
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Hemípteros
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article