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Development, clinical translation, and utility of a COVID-19 antibody test with qualitative and quantitative readouts.
Bortz, Robert H; Florez, Catalina; Laudermilch, Ethan; Wirchnianski, Ariel S; Lasso, Gorka; Malonis, Ryan J; Georgiev, George I; Vergnolle, Olivia; Herrera, Natalia G; Morano, Nicholas C; Campbell, Sean T; Orner, Erika P; Mengotto, Amanda; Dieterle, M Eugenia; Fels, J Maximilian; Haslwanter, Denise; Jangra, Rohit K; Celikgil, Alev; Kimmel, Duncan; Lee, James H; Mariano, Margarette; Antonio, Nakouzi; Jose, Quiroz; Rivera, Johanna; Szymczak, Wendy A; Tong, Karen; Barnhill, Jason; Forsell, Mattias N E; Ahlm, Clas; Stein, Daniel T; Pirofski, Liise-Anne; Goldstein, D Yitzchak; Garforth, Scott J; Almo, Steven C; Daily, Johanna P; Prystowsky, Michael B; Faix, James D; Fox, Amy S; Weiss, Louis M; Lai, Jonathan R; Chandran, Kartik.
Afiliação
  • Bortz RH; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Florez C; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Laudermilch E; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY 10996, USA.
  • Wirchnianski AS; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Lasso G; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Malonis RJ; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Georgiev GI; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Vergnolle O; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Herrera NG; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Morano NC; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Campbell ST; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Orner EP; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Mengotto A; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Dieterle ME; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Fels JM; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Haslwanter D; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Jangra RK; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Celikgil A; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Kimmel D; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Lee JH; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Mariano M; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Antonio N; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Jose Q; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Rivera J; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Szymczak WA; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Tong K; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Barnhill J; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Forsell MNE; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Ahlm C; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Stein DT; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Pirofski LA; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, NY 10996, USA.
  • Goldstein DY; Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
  • Garforth SJ; Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
  • Almo SC; Division of Endocrinology & Diabetes, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Daily JP; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Prystowsky MB; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
  • Faix JD; Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Fox AS; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Weiss LM; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Lai JR; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
  • Chandran K; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY 10461, USA.
medRxiv ; 2020 Sep 11.
Article em En | MEDLINE | ID: mdl-32935116
The COVID-19 global pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to place an immense burden on societies and healthcare systems. A key component of COVID-19 control efforts is serologic testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity. We further show that this test affords the estimation of viral spike-specific IgG titers from a single sample measurement, thereby providing a simple and scalable method to measure the strength of an individual's immune response. The accuracy, adaptability, and cost-effectiveness of this test makes it an excellent option for clinical deployment in the ongoing COVID-19 pandemic.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Qualitative_research / Risk_factors_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Qualitative_research / Risk_factors_studies Idioma: En Ano de publicação: 2020 Tipo de documento: Article