Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes.

Siris, Sara; Gladstone, Camilla A; Guo, Yanping; Pinder, Christopher L; Shattock, Robin J; McKay, Paul F; Langford, Paul R; Bidmos, Fadil A

Siris, Sara; Gladstone, Camilla A; Guo, Yanping; Pinder, Christopher L; Shattock, Robin J; McKay, Paul F; Langford, Paul R; Bidmos, Fadil A.

Afiliação

Siris S; Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, UK.
Gladstone CA; Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, UK.
Guo Y; Flow Cytometry Core Facility, National Heart and Lung Institute, Imperial College London, London, UK.
Pinder CL; Section of Immunology of Infection, Department of Infectious Disease, Imperial College London, London, UK.
Shattock RJ; Section of Immunology of Infection, Department of Infectious Disease, Imperial College London, London, UK.
McKay PF; Section of Immunology of Infection, Department of Infectious Disease, Imperial College London, London, UK.
Langford PR; Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, UK.
Bidmos FA; Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, London, UK. f.bidmos@imperial.ac.uk.

Methods Mol Biol ; 2183: 9-18, 2021.

Article em En | MEDLINE | ID: mdl-32959237

Assuntos

Anticorpos Monoclonais/imunologia; Anticorpos Monoclonais/isolamento & purificação; Especificidade de Anticorpos/imunologia; Bactérias/imunologia; Imunoensaio/métodos; Sondas Moleculares; Anticorpos Antibacterianos/imunologia; Afinidade de Anticorpos; Formação de Anticorpos/imunologia; Antígenos de Bactérias/imunologia; Interações Hospedeiro-Patógeno; Humanos; Imunoglobulina G/imunologia; Plasmócitos/imunologia; Plasmócitos/metabolismo; Streptococcus pneumoniae/imunologia

Palavras-chave

Bacteria; Immunoglobulin capture assay; Pathogen-specific plasmablasts; Vaccine antigen discovery; Whole cells

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Imunoensaio / Sondas Moleculares / Anticorpos Monoclonais / Especificidade de Anticorpos Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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