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Coupled intra- and interdomain dynamics support domain cross-talk in Pin1.
Zhang, Meiling; Frederick, Thomas E; VanPelt, Jamie; Case, David A; Peng, Jeffrey W.
Afiliação
  • Zhang M; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA.
  • Frederick TE; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA.
  • VanPelt J; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA.
  • Case DA; Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey, USA.
  • Peng JW; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA. Electronic address: jpeng@nd.edu.
J Biol Chem ; 295(49): 16585-16603, 2020 12 04.
Article em En | MEDLINE | ID: mdl-32963105
ABSTRACT
The functional mechanisms of multidomain proteins often exploit interdomain interactions, or "cross-talk." An example is human Pin1, an essential mitotic regulator consisting of a Trp-Trp (WW) domain flexibly tethered to a peptidyl-prolyl isomerase (PPIase) domain, resulting in interdomain interactions important for Pin1 function. Substrate binding to the WW domain alters its transient contacts with the PPIase domain via means that are only partially understood. Accordingly, we have investigated Pin1 interdomain interactions using NMR paramagnetic relaxation enhancement (PRE) and molecular dynamics (MD) simulations. The PREs show that apo-Pin1 samples interdomain contacts beyond the range suggested by previous structural studies. They further show that substrate binding to the WW domain simultaneously alters interdomain separation and the internal conformation of the WW domain. A 4.5-µs all-atom MD simulation of apo-Pin1 suggests that the fluctuations of interdomain distances are correlated with fluctuations of WW domain interresidue contacts involved in substrate binding. Thus, the interdomain/WW domain conformations sampled by apo-Pin1 may already include a range of conformations appropriate for binding Pin1's numerous substrates. The proposed coupling between intra-/interdomain conformational fluctuations is a consequence of the dynamic modular architecture of Pin1. Such modular architecture is common among cell-cycle proteins; thus, the WW-PPIase domain cross-talk mechanisms of Pin1 may be relevant for their mechanisms as well.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptidilprolil Isomerase de Interação com NIMA Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptidilprolil Isomerase de Interação com NIMA Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article