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Strain-specific strategies of 2'-fucosyllactose, 3-fucosyllactose, and difucosyllactose assimilation by Bifidobacterium longum subsp. infantis Bi-26 and ATCC 15697.
Zabel, Bryan E; Gerdes, Svetlana; Evans, Kara C; Nedveck, Derek; Singles, Suzanne Koch; Volk, Barbara; Budinoff, Charles.
Afiliação
  • Zabel BE; Genomics and Microbiome Science, DuPont Nutrition and Biosciences, Madison, WI, USA. bryan.zabel@dupont.com.
  • Gerdes S; Genomics and Microbiome Science, DuPont Nutrition and Biosciences, Madison, WI, USA.
  • Evans KC; Genomics and Microbiome Science, DuPont Nutrition and Biosciences, Madison, WI, USA.
  • Nedveck D; Genomics and Microbiome Science, DuPont Nutrition and Biosciences, Madison, WI, USA.
  • Singles SK; Advanced Analytical, DuPont Nutrition and Biosciences, Wilmington, DE, USA.
  • Volk B; Advanced Analytical, DuPont Nutrition and Biosciences, Wilmington, DE, USA.
  • Budinoff C; Genomics and Microbiome Science, DuPont Nutrition and Biosciences, Madison, WI, USA.
Sci Rep ; 10(1): 15919, 2020 09 28.
Article em En | MEDLINE | ID: mdl-32985563
ABSTRACT
Human milk provides essential nutrients for infant nutrition. A large proportion of human milk is composed of human milk oligosaccharides (HMOs), which are resistant to digestion by the infant. Instead, HMOs act as a bioactive and prebiotic enriching HMO-utilizing bacteria and cause systematic changes in the host. Several species of Bifidobacterium have been shown to utilize HMOs by conserved, as well as species-specific pathways, but less work has been done to study variation within species or sub-species. B. longum subsp. infantis is a prevalent species in the breast-fed infant gut and the molecular mechanisms of HMO utilization for the type strain B. longum subsp. infantis ATCC 15697 (type strain) have been well characterized. We used growth, transcriptomic, and metabolite analysis to characterize key differences in the utilization of 2'FL, 3FL and DFL (FLs) between B. longum subsp. infantis Bi-26 (Bi-26) and the type strain. Bi-26 grows faster, produces unique metabolites, and has a distinct global gene transcription response to FLs compared to the type strain. Taken together the findings demonstrate major strain specific adaptations in Bi-26 to efficient utilization of FLs.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Trissacarídeos / Trato Gastrointestinal / Bifidobacterium longum Limite: Humans / Infant Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Trissacarídeos / Trato Gastrointestinal / Bifidobacterium longum Limite: Humans / Infant Idioma: En Ano de publicação: 2020 Tipo de documento: Article