Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics.
Analyst
; 145(23): 7718-7723, 2020 Nov 23.
Article
em En
| MEDLINE
| ID: mdl-32996917
ABSTRACT
We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme ß-galactosidase and substrate 4-methylumbelliferyl ß-d-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microfluídica
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article