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Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step.
Bruce, Emily A; Huang, Meei-Li; Perchetti, Garrett A; Tighe, Scott; Laaguiby, Pheobe; Hoffman, Jessica J; Gerrard, Diana L; Nalla, Arun K; Wei, Yulun; Greninger, Alexander L; Diehl, Sean A; Shirley, David J; Leonard, Debra G B; Huston, Christopher D; Kirkpatrick, Beth D; Dragon, Julie A; Crothers, Jessica W; Jerome, Keith R; Botten, Jason W.
Afiliação
  • Bruce EA; Division of Immunobiology, Department of Medicine, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Huang ML; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America.
  • Perchetti GA; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America.
  • Tighe S; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Laaguiby P; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Hoffman JJ; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Gerrard DL; Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington, Vermont, United States of America.
  • Nalla AK; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America.
  • Wei Y; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America.
  • Greninger AL; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, United States of America.
  • Diehl SA; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.
  • Shirley DJ; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Leonard DGB; Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Huston CD; Data Science Division, IXIS, Burlington, Vermont, United States of America.
  • Kirkpatrick BD; Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Dragon JA; University of Vermont Health Network, Burlington, Vermont, United States of America.
  • Crothers JW; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
  • Jerome KR; Division of Infectious Disease, Department of Medicine, University of Vermont Medical Center, Burlington, Vermont, United States of America.
  • Botten JW; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, Vermont, United States of America.
PLoS Biol ; 18(10): e3000896, 2020 10.
Article em En | MEDLINE | ID: mdl-33006983
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Kit de Reagentes para Diagnóstico / RNA Viral / Infecções por Coronavirus / Técnicas de Laboratório Clínico / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Betacoronavirus Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans País/Região como assunto: America do norte Idioma: En Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Kit de Reagentes para Diagnóstico / RNA Viral / Infecções por Coronavirus / Técnicas de Laboratório Clínico / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Betacoronavirus Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans País/Região como assunto: America do norte Idioma: En Ano de publicação: 2020 Tipo de documento: Article