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G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells.
Richardson, Adam E; Zentz, Zachary A; Chambers, Antonio E; Sandwith, Siara N; Reisinger, Michael A; Saunders, Destinee W; Tompkins, Joshua D; Riggs, Arthur D; Routh, Eric D; Rubenstein, Eric M; Smaldino, Melissa A; Vaughn, James P; Haney, Robert A; Smaldino, Philip J.
Afiliação
  • Richardson AE; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Zentz ZA; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Chambers AE; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Sandwith SN; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Reisinger MA; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Saunders DW; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Tompkins JD; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, California 91010, United States.
  • Riggs AD; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, California 91010, United States.
  • Routh ED; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
  • Rubenstein EM; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Smaldino MA; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Vaughn JP; NanoMedica LLC, Winston-Salem, North Carolina 27101, United States.
  • Haney RA; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
  • Smaldino PJ; Department of Biology, Ball State University, Muncie, Indiana 47306, United States.
ACS Omega ; 5(38): 24916-24926, 2020 Sep 29.
Article em En | MEDLINE | ID: mdl-33015511
G-quadruplexes (G4s) are nucleic acid structures found enriched within gene regulatory sequences. G4s control fundamental cellular processes, including replication, transcription, and translation. Proto-oncogenes are enriched with G4 sequences, while tumor-suppressor genes are depleted, suggesting roles for G4s in cell survival and proliferation. Specialized helicases participate in G4-mediated gene regulation via enzymatic unwinding activity. One such enzyme, DHX36/G4R1, is the major G4-helicase and is a master regulator of G4-DNAs and mRNAs. G4-resolution promotes the expression of proproliferative genes; as such, DHX36/G4R1 promotes cell proliferation. Little is known about how DHX36/G4R1 itself is regulated in nondividing cells. We hypothesized that DHX36/G4R1 protein binding partners are altered when a cell transitions from a dividing to a quiescent state. We found that DHX36/G4R1 co-purifies with a distinct set of proteins under quiescent conditions, which may represent a novel complex that regulates DHX36/G4R1 during cell cycle transitions and have implications for development and cancer.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2020 Tipo de documento: Article